关键词: 非小细胞肺癌, lncRNA B3GALT5-AS1, 克隆形成, Transwell, 细胞凋亡

Abstract: Objective To explore the effect of overexpression of long non-coding RNA ( lncRNA) B3GALT5-AS1 on the growth and invasion ability in non-small cell lung cancer (NSCLC).Methods qRT-PCR was employed to examine the expression level of B3GALT5-AS1 in clinicalsamples. A549 cells were divided into 3 groups: control group, pcDNA-scramble group, and pcDNA-B3GALT5-AS1 group, the pcDNA-scramble and pcDNA-B3GALT5 AS1 vectors were transfected into cells respectively. The colony formation experiment was employed to evaluate the colony-forming ability of A549 cells, qRT-PCR was used to detect the mRNA expression level of Ki67 and Survivin. Flow cytometry was used to analyse cell apoptosis, Transwell assay was used to test cell invasion, and Western blotting was used to detect the expression level of P27, P53, VEGF and Vimentin proteins. A549 cells transfected with pcDNA-scramble or pcDNA-B3GALT5-AS1 were injected subcutaneously into nude mice. The tumor volume and weight were measured, the expression level of B3GALT5-AS1 in tumor tissues was detected by qRT-PCR, and the expression of Ki67 and VEGF were determinded by immunohistochemistry. Results The relative mRNA expression level ofB3GALT5-AS1 in the lung cancer tissues was significantly lower than that in the adjacent normal tissues when compared with that in the paracancerous tissues (P< 0. 05). The colony forming ability of cells in the pcDNA-B3GALT5-AS1 group was reduced ( P < 0. 05), the expression of Ki67 and Survivin was obviously down-regulated (P< 0. 05), the apoptosis rate of cell was clearly increased (P< 0. 05), and the number of invasion cells was evidently reduced (P < 0. 05), the expression level of P27 and P53 proteins was up-regulated (P< 0. 05), and the expression of VEGF and Vimentin protein was significantly down-regulated (P< 0. 05). The results of in vivo xenotransplantationexperiments showed that overexpression of B3GALT5-AS1 clearly reduced tumor volume and weight(P< 0. 05), and the numbers of Ki67 and VEGF positive cells in tumor tissues were obviously reduced (P< 0. 05) when compared with that in the pcDNA-scramble group. Conclusion Overexpression of B3GALT5-AS1 obviously inhibits the proliferation and invasion of NSCLC cells and induces apoptosis, it also blocks the growth of tumor tissues in vivo and plays an anti-cancer effect in NSCLC.

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