关键词: 积雪草苷, 慢性粒细胞白血病, 伊马替尼, 迁移, GATA-1

Abstract: Objective To study the effect of asiaticoside on the proliferation, invasion and cellcycle arrest of chronic myelogenous leukemia (CML) cells, and to explore its regulation on GATA-1 expression. Methods CML cell line K562, imatinib (IM) -resistant K562 cells (K562r) andhuman original CML cells were used for experiments. Cell viability was determined by CCK-8 assay. K562r cells were treated with 0, 25, 50, 100 μmol / L asiaticoside, then cell proliferation wasdetermined by colony formation assay, cell migration was determined by transwell assay, the cell cycle was measured by flow cytometry, the expression levels of cyclin A, CDK2, CDK4, cyclinD1, Ki67, PCNA, VEGF, MMP-9 and MMP-14 were detected by Western blotting. Results Compared with the control group, asiaticoside treatment significantly reduced the number of K562rcell colonies and migration cells, and induced the cell cycle arrest. In addition, the expression levelof GATA-1 in K562r cells was significantly up-regulated after asiaticoside treatment. Conclusion Asiaticoside can inhibit the proliferation and migration of K562r cells and induce cell cycle arrest,and the mechanism may be related to the up-regulation of GATA-1 expression.

Key words: asiaticoside, chronic myelogenous leukemia, imatinib, cell migration, GATA-1