Interference of lncRNA NCK1-AS1 Experssion Inhibits Growth and Invasion of CAL-62 Thyroid Cancer Cells

doi:10.3870/j.issn.1672-8009.2026.04.008

Abstract

Abstract: Objective To investigate the effects of interfering with the expression of the lncRNA NCK1-AS1 on the growth and invasion of the thyroid cancer cell line CAL-62. Methods lncRNA NCK1-AS1 expression in CAL-62 cells was silenced by transfecting the corresponding shRNA.The expression level of lncRNA NCK1-AS1 was detected by RT-PCR.Cell proliferation was assessed by EdU staining,and apoptosis was analyzed by flow cytometry.RT-PCR was used to detect the mRNA expression levels of proliferating cell nuclear antigen(PCNA),p21,and Survivin.Cell invasion was assessed using the Transwell assay,and the number of microtubule-like structures was measured via a microtubule formation assay.Western blot analysis was performed to detect the expression levels of vascular endothelial growth factor(VEGF),E-cadherin,and N-cadherin. Results Compared with the control group,the lncRNA NCK1-AS1 interference group exhibited reduced cell proliferation,increased apoptosis,downregulated PCNA and Survivin mRNA levels,upregulated P21 mRNA levels,decreased number of invading cells and microtubule-like structures,downregulated VEGF and N-cadherin protein expression,and upregulated E-cadherin expression.These differences were statistically significant(P<0.05). Conclusion Silencing the expression of lncRNA NCK1-AS1 inhibits the growth and invasion of CAL-62 thyroid cancer cells.

Key words: lncRNA NCK1-AS1, CAL-62 cells, cell growth, exercise capacity

CLC Number:

WANG Dan, WANG Qiong, ZHAO Qian, XU Lin, LIU Tianyu, SUN Jirui. Interference of lncRNA NCK1-AS1 Experssion Inhibits Growth and Invasion of CAL-62 Thyroid Cancer Cells[J]. Journal of Medical Molecular Biology, 2026, 23(4): 424-430.

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