Abstract: Objective To explore the effect of lncRNA CERS6-AS1 on the biological behavior of glioma cells and its possible mechanism. Methods qRT-PCR method was used to detect the expression levels of CERS6-AS1 and miR-138-2-3p in glioma tissues and adjacent tissues. Pearson method was used to analyze the correlation between CERS6-AS1 and miR-138-2-3p expression in glioma tissues. Human glioma cells T98G were cultured in vitro, and then were transfected with si-NC, si-CERS6-AS1, miR-NC, miR-138-2-3p mimics, si-CERS6-AS1 and anti-miR-NC, si-CERS6-AS1 and anti-miR-138-2-3p. The CCK-8 method, plate colony formation experiment, and transwell assay were used to detect the cell capabilities of proliferation, clone formation, migration and invasion. The dual luciferase reporter gene experiment was used to verify the targeting relationship between CERS6-AS1 and miR-138-2-3p. Results Compared with the adjacent tissues, the expression level ofCERS6-AS1 in glioma tissues was increased (P< 0. 05), while the expression level of miR-138-2-3p was decreased (P< 0. 05). CERS6-AS1 was negatively correlated with miR-138-2-3p (r = - 0. 8899, P< 0. 001). CERS6-AS1 could target the expression of miR-138-2-3p. The cell viability, the number of colony forming cells, the number of migration and invasion cells in the si-CERS6-AS1 group were lower than those in the si-NC group (P< 0. 05). The cell viability, the number of colony formed cells, the number of migrated and invasive cells in the miR-138-2-3p group were less than those in the miR-NC group (P< 0. 05). The cell viability, the number of clone formed cells, the number of migrated and invasive cells in the si-CERS6-AS1 + anti-miR-138-2-3p group were all higher than those in the si-CERS6-AS1 + anti-miR-NC group (P< 0. 05). Conclusion Interference of the expression of CERS6-AS1 could inhibit the proliferation, clone formation, migration and invasion of glioma cells by regulating miR-138-2-3p.

Key words: lncRNA CERS6-AS1, miR-138-2-3p, glioma, cell proliferation, invasion