Long Non-Coding RNA-mRNA Regulatory Networks Involved in the Pathogenesis of Acute Coronary Syndrome and Their Impact on Key Signaling Pathways

doi:10.3870/j.issn.1672-8009.2026.03.006

Abstract

Abstract: Objective To identify key molecular biomarkers of acute coronary syndrome(ACS)through transcriptome sequencing and construction of mRNA-lncRNA co-expression networks. Methods An ACS mouse model was established,and the modeling outcomes and cardiac pathological changes were comprehensively evaluated using physiological and pathological assays.RNA sequencing(RNA-seq)was performed to obtain gene expression profiles across different samples.Bioinformatics analysis was then applied to explore gene expression changes and functional signaling pathways associated with ACS. Results After ACS modeling,experimental results such as HE staining,TTC staining,and Doppler ultrasound all indicated that ACS led to histopathological changes and dysfunction in mouse cardiac tissue.RNA-seq results revealed 2 557 DEGs that were co-upregulated in both ACS-M and ACS-S,and 1 930 DEGs that were co-downregulated,including mRNAs(DEmRNAs)and lncRNAs(DElncRNAs).Further GO functional enrichment analysis showed that these DElncRNAs regulated the differential expression of target genes related to mitochondrial function,fatty acid metabolism,immune/inflammatory response,cell adhesion,apoptosis,fibrosis,and other functions.Additionally,six expression patterns closely associated with ACS were identified.Among them,lncRNAs Dnm3os and Gm13054 were persistently highly expressed in ACS and their high expression characteristics were validated by RT-qPCR.Their downstream targets were closely related to mitochondrial function and tissue fibrosis.Gm13054 also exhibited potential regulatory functions for Nppa expression. Conclusion This study identified a large number of DEmRNAs and DElncRNAs through RNA-seq,which have potential functions tightly associated with ACS.The construction of a co-expression network between mRNAs and lncRNAs provides potential therapeutic targets and a foundational basis for further clinical research on the diagnosis and treatment of ACS.

Key words: acute coronary syndrome, transcriptome sequencing, long non-coding RNA, differentially expressed genes

CLC Number:

R541.1

QUAN Youwu, CHEN Yuanya, WU Zhengjie, LIU Kaixiong, YE Yuanzheng. Long Non-Coding RNA-mRNA Regulatory Networks Involved in the Pathogenesis of Acute Coronary Syndrome and Their Impact on Key Signaling Pathways[J]. Journal of Medical Molecular Biology, 2026, 23(3): 275-284.

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