一氧化氮动态检测在食管鳞癌细胞与类器官体外鉴别及化疗反应初步评估中的应用探索*

doi:10.3870/j.issn.1672-0741.26.04.024

华中科技大学学报（医学版） ›› 2026, Vol. 55 ›› Issue (5): 651-658.doi: 10.3870/j.issn.1672-0741.26.04.024

一氧化氮动态检测在食管鳞癌细胞与类器官体外鉴别及化疗反应初步评估中的应用探索^*

姚开元^1,2, 高社干^1,2, 赵安顺^1,2△

¹河南科技大学临床医学院,河南科技大学第一附属医院,河南省肿瘤表观遗传重点实验室, 河南省微生态与食管癌防治重点实验室,洛阳 471003;
²河南省科学院类器官芯片与药物转化研究院,郑州 450001

收稿日期:2026-04-25 发布日期:2026-10-30
通讯作者: ^△E-mail：anshunzhao@haust.edu.cn
作者简介:姚开元,男,2000年生,硕士研究生,E-mail：230320201168@stu.haust.edu.cn
基金资助:
^*国家自然科学基金资助项目（No.82202644）

Dynamic Nitric Oxide Monitoring for in vitro Discrimination of Esophageal Squamous Cell Carcinoma Cells and Organoids and Preliminary Assessment of Chemotherapy Response

Yao Kaiyuan^1,2, Gao Shegan^1,2, Zhao Anshun^1,2△

¹Key Laboratory of Microbiome and Esophageal Cancer Prevention and Treatment, Henan Key Laboratory of Cancer Epigenetics, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang 471003, China;
²Institute of Organoid on Chip and Drug Translation Research, Henan Academy of Sciences, Zhengzhou 450001, China

Received:2026-04-25 Published:2026-10-30
Contact: ^△E-mail：anshunzhao@haust.edu.cn

摘要/Abstract

摘要： 目的在细胞与类器官水平,探讨一氧化氮（nitric oxide,NO）动态检测用于食管鳞癌（esophageal squamous cell carcinoma,ESCC）与正常食管上皮细胞鉴别以及化疗药物反应初步评估的可行性。方法采用电化学传感器实时检测ESCC细胞系KYSE30、KYSE150及正常食管上皮细胞NE6-T在L-精氨酸刺激下的NO释放水平;构建ESCC细胞及类器官体外化疗模型,检测紫杉醇（paclitaxel,PTX）处理后NO的释放变化,并将NO信号换算的相对活力与CCK-8法结果进行Pearson相关性分析。结果 ESCC细胞NO水平高于正常食管上皮细胞（P<0.05）,其升高可能与HIF-1α-iNOS通路激活相关;PTX处理后,NO电化学检测法与CCK-8法所得的细胞活力正相关（r=0.9579,P<0.05）,两种方法检测所得类器官活力亦呈正相关（r=0.9116,P<0.05）,表明两种检测方法具有良好的一致性。结论 NO动态释放特征可在体外水平区分ESCC细胞与正常食管上皮细胞,并能反映化疗药物处理后肿瘤细胞及类器官的活力变化。NO动态检测在ESCC体外鉴别与药敏筛选中具有一定价值。

关键词: 食管鳞状细胞癌, 一氧化氮, 化疗敏感性, 类器官, HIF-1α

Abstract: Objective this study investigated the feasibility of real-time nitric oxide （NO） monitoring for distinguishing between esophageal squamous cell carcinoma （ESCC） and normal esophageal epithelial cells at the cellular and organoid levels, and for preliminary assessment of chemotherapy response. Methods An electrochemical sensor was used to monitor NO release levels in the ESCC cell lines KYSE30 and KYSE150, as well as in normal esophageal epithelial cells （NE6-T）, upon L-arginine stimulation.An in vitro chemotherapy model with ESCC cells and organoids was established to assess changes in NO release after paclitaxel （PTX） treatment. Pearson correlation analysis was performed between relative viability derived from NO signals and that obtained via the CCK-8 assay. Results NO levels in ESCC cells were higher than those in normal esophageal epithelial cells （P<0.05）, and this elevation may be associated with the activation of the HIF-1α-iNOS pathway. Following PTX treatment, cell viability estimated from NO measurements showed a strong positive correlation with that determined by the CCK-8 assay （r= 0.9579, P<0.05）, and organoid viability also showed a strong positive correlation （r=0.9116, P<0.05）, indicating good consistency between the two methods. Conclusion Dynamic NO release can distinguish ESCC cells from normal esophageal epithelial cells in vitro and reflect changes in the viability of tumor cells and organoids following treatment with chemotherapeutic agents. This suggests that dynamic NO detection holds value in the in vitro discrimination of ESCC and drug sensitivity screening.

Key words: ESCC, nitric oxide, chemotherapy sensitivity, organoid, HIF-1α

中图分类号:

R735.1

姚开元, 高社干, 赵安顺. 一氧化氮动态检测在食管鳞癌细胞与类器官体外鉴别及化疗反应初步评估中的应用探索^*[J]. 华中科技大学学报（医学版）, 2026, 55(5): 651-658.

Yao Kaiyuan, Gao Shegan, Zhao Anshun. Dynamic Nitric Oxide Monitoring for in vitro Discrimination of Esophageal Squamous Cell Carcinoma Cells and Organoids and Preliminary Assessment of Chemotherapy Response[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(5): 651-658.

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一氧化氮动态检测在食管鳞癌细胞与类器官体外鉴别及化疗反应初步评估中的应用探索^*

Dynamic Nitric Oxide Monitoring for in vitro Discrimination of Esophageal Squamous Cell Carcinoma Cells and Organoids and Preliminary Assessment of Chemotherapy Response

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