紫花前胡素通过miR-17-5p靶向PI3K/Akt通路对结直肠癌细胞增殖和迁移的影响*

doi:10.3870/j.issn.1672-0741.24.08.036

摘要/Abstract

摘要： 目的探讨紫花前胡素(Decursin,DE)对结直肠癌细胞增殖和迁移的影响及其具体机制。方法选取SW480、HCoEpiC细胞进行实验,qRT-PCR法检测两种细胞系中miR-17-5p表达水平。CCK-8法检测不同DE浓度梯度(0、10、30、60和100 μmol/L)下SW480细胞增殖活性。qRT-PCR法检测SW480细胞中miR-17-5p(mimic/inhibitor)的转染效率,CCK-8法检测转染后细胞增殖活性,Transwell实验检测迁移细胞数。建立转染(mimic/inhibitor)组与转染(mimic/inhibitor)＋DE组SW480细胞,采用qRT-PCR法检测miR-17-5p表达量变化,CCK-8法检测细胞增殖活性,Transwell实验检测迁移细胞数,Western blot法检测PIK3R1、p-PI3K、PI3K、p-Akt、Akt蛋白表达量变化。双荧光素酶报告基因实验检测miR-17-5p与PI3K的靶向作用。结果 SW480细胞miR-17-5p表达水平高于HCoEpiC细胞(P＜0.05)。SW480细胞增殖活性随DE处理浓度的升高呈现浓度依赖性降低(P＜0.05);选择60 μmol/L DE用于后续实验。miR-17-5p mimic/inhibitor可显著升高/降低SW480细胞中miR-17-5p的表达量、细胞增殖活性和细胞迁移数(均P＜0.05)。加入DE后各组miR-17-5p表达量、细胞增殖活性、细胞迁移数以及p-PI3K和p-Akt蛋白表达量降低(均P＜0.05),PIK3R1蛋白表达量升高(P＜0.05),PI3K、Akt蛋白表达量无明显变化。共转染miR-17-5p与Wt-PIK3R1,荧光素酶相对活性随miR-17-5p表达量的升高/降低而降低/升高(均P＜0.05)。共转染miR-17-5p与Mut-PIK3R1,荧光素酶相对活性无显著变化(P＞0.05)。结论 DE可抑制结直肠癌细胞增殖和迁移,其机制可能是通过下调miR-17-5p从而促进PIK3R1表达,抑制PI3K/Akt通路发挥作用。

关键词: 结直肠癌, 紫花前胡素, 细胞增殖, 细胞迁移, miR-17-5p

Abstract: Objective To investigate the effects of Decursin(DE)on the proliferation and migration of colorectal cancer cells and the underlying mechanism. Methods Third-generation SW480 and HCoEpiC cells were selected for the experiment,and the expression of miR-17-5p RNA in the two cell lines was detected via qRT-PCR.The proliferation of SW480 cells was detected via the CCK-8 method at different DE concentrations(0,10,30,60 and 100 μmol/L),and the appropriate concentration of DE was selected.qRT-PCR was used to detect the transfection efficiency of miR-17-5p in SW480 cells.The proliferation of the transfected cells was detected via CCK-8 method,and the number of migrating cells was detected via Transwell experiment.The expression of miR-17-5p was detected via qRT-PCR,the cell proliferation activity was detected via the CCK-8 method,the number of migrating cells was detected via Transwell experiment,and the levels of PIK3R1,p-PI3K,PI3K and p-Akt were detected via Western blotting.The dual-luciferase reporter assay was used to detect the targeting effect of miR-17-5p on PI3K. Results The expression level of miR-17-5p RNA in W480 cells was greater than that in HCoEpiC cells(P<0.05).The proliferation of SW480 cells decreased with increasing DE concentration in a concentration-dependent manner(P<0.05).For the subsequent experiments,60 μmol/L DE was selected.The miR-17-5p mimic/inhibitor significantly increased/decreased the expression of miR-17-5p,cell proliferation activity and the number of migrating cells(P<0.05).After treatment with DE,the expression of miR-17-5p,cell proliferation activity,cell migration,and p-PI3K and p-Akt proteins decreased(P<0.05),whereas the expression of the PIK3R1 protein increased(P<0.05);however,the expression of the PI3K and Akt proteins did not change significantly.When miR-17-5p and Wt-PIK3R1 were co-transfected,the relative luciferase activity decreased/increased with increasing/decreasing miR-17-5p expression(P<0.05).When miR-17-5p and Mut-Wt-PIK3R1 were co-transfected,the relative activity of luciferase did not change significantly(P> 0.05). Conclusion Decursin can inhibit the proliferation and migration of colorectal cancer cells,which may inhibit the PI3K/Akt pathway by downregulating miR-17-5p and promoting the expression of PIK3R1.

Key words: colorectal cancer, Decursin, cell proliferation, cell migration, miR-17-5p

中图分类号:

R735.3

吕九娣, 彭昕, 邢玉广, 王俊杰, 胡军红. 紫花前胡素通过miR-17-5p靶向PI3K/Akt通路对结直肠癌细胞增殖和迁移的影响^*[J]. 华中科技大学学报（医学版）, 2026, 55(1): 76-81.

Lv Jiudi, Peng Xin, Xing Yuguang et al. Effects of Decursin on Proliferation and Migration of Colorectal Cancer Cells via Upregulation of miR-17-5p through Targeted Regulation of PI3K/Akt Pathway[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(1): 76-81.

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紫花前胡素通过miR-17-5p靶向PI3K/Akt通路对结直肠癌细胞增殖和迁移的影响^*

Effects of Decursin on Proliferation and Migration of Colorectal Cancer Cells via Upregulation of miR-17-5p through Targeted Regulation of PI3K/Akt Pathway

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