Abstract: Objective To investigate the effects and mechanisms of LINC00365 on proliferation, apoptosis, migration and invasion of thyroid cancer cells. Methods qRT-PCR was used todetect the relative expression level of LINC00365 in thyroid cancer tissues and thyroid cancer celllines. Thyroid cancer cells BHP-10-3 were divided into 5 groups: siNC group, si-LINC00365 group, si-LINC00365 + NC inhibitor group, si-LINC00365 + miR-519d inhibitor group, and blank group. Dual luciferase reporter gene experiment was used to detect the targeting relationship between LINC00365 and miR-519d. Cell proliferation ability was detected by CCK8, cell migration abilitywas detected by wound healing assay, cell invasion ability was detected by transwell assay, apoptosis rate was detected by flow cytometry. Results The relative expression level of LINC00365 were significantly upregulated in thyroid cancer tissues and thyroid cancer cells. miR-519d is the target gene of LINC00365. the proliferation, migration, and invasion abilities of BHP-10-3 in the siLINC00365 group and the si-LINC00365 + NC inhibitor group were significantly reduced ( P <0. 05), and the cell apoptosis rate was significantly increased ( P < 0. 01) when compared withthose in the blank group. The proliferation, migration, and invasion abilities of BHP-10-3 in the siLINC00365 + miR-519d inhibitor group were significantly increased (P < 0. 05), and the cell apoptosis rate was significantly reduced (P < 0. 01) when compared with those in the si-LINC00365 group. Conclusion LINC00365 can target miR-519d to affect the proliferation, migration, invasion and apoptosis of thyroid cancer cells.

Key words: LINC00365, thyroid cancer, proliferation, apoptosis, migration, invasion