Effect of ULK1 on Invasion and Migration of Placental Trophoblastic Cells of Preeclampsia Rats via Wnt Signaling Pathway

doi:10.3870/j.issn.1672-8009.2026.04.006

Abstract

Abstract: Objective The effect of unc-51 like kinase 1(ULK1)on the invasion and migration of trophoblastic cells in preeclampsia rats was analyzed based on the Wnt signaling pathway. Methods After establishing a preeclampsia rat model,trophoblastic cells were isolated from placental tissues.Preeclampsia trophoblastic cells were randomly divided into 4 groups:negative control group,siRNA ULK1 group,SKL2001 group,and siRNA ULK1+KYA1797K group.The proliferation activity of trophoblastic cells was detected by CCK8 assay;the invasion and migration abilities of trophoblastic cells were detected by Transwell assay;the apoptosis rate of trophoblastic cells was detected by flow cytometry;the expression levels of ULK1 and Wnt pathway proteins in trophoblastic cells were analyzed by Western blotting. Results The expression level of ULK1 in trophoblastic cells of the siRNA ULK1 group and the siRNA ULK1+KYA1797K group was significantly decreased,while the expression level of ULK1 in the SKL2001 group showed no significant change.The proliferation activity of trophoblastic cells in the siRNA ULK1 and SKL2001 groups was increased,their invasion and migration abilities were enhanced,the apoptosis rate was decreased,and the protein expression levels of Wnt3α,β-catenin,c-Myc,and Cyclin D1 were upregulated.In the siRNA ULK1+KYA1797K group,the proliferation activity,invasion and migration abilities,apoptosis rate,and protein expression levels of Wnt3α,β-catenin,c-Myc,and Cyclin D1 showed no significant changes compared with those in the the negative control group. Conclusion Inhibition of ULK1 expression increases the proliferation,migration and invasion activities of trophoblastic cells in preeclampsia rats and reduces the apoptosis rate,and these processes are related to the activation of the Wnt/β-catenin pathway.

Key words: unc-51 like kinase 1, Wnt signaling pathway, trophoblastic cell, invasion, migration, apoptosis

CLC Number:

R714.2

DING Shu, SU Gendi, ZHANG Linyan, JIANG Wenjing. Effect of ULK1 on Invasion and Migration of Placental Trophoblastic Cells of Preeclampsia Rats via Wnt Signaling Pathway[J]. Journal of Medical Molecular Biology, 2026, 23(4): 412-417.

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