Abstract: Objective To explore the application value of gene detection of miR-495-3p andmanganese superoxide dismutase (Mn-SOD) in the disease evaluation of diabetes nephropathy patients. Methods A total of 150 patients with diabetes nephropathy who were treated in HuangshiCentral Hospital from January 2022 to December 2023 were selected, and another 150 patients with diabetes and 150 healthy people were included in this study as controls. the differences of miR-495-3p expression and Mn-SOD genotypes in each group were compared, and the value of miR-495-3p expression and Mn-SOD genotypes in predicting poor prognosis of diabetes nephropathy were analyzed. Results The expression level of miR-495-3p in the group of diabetes nephropathy patients was (0. 56 ± 0. 14), which was significantly lower than that of diabetes and healthy people (P<0. 05). There was a statistically significant difference in the distribution of genotypes at the 9 and5 777 loci of Mn-SOD gene in diabetes nephropathy, diabetes and healthy people (P< 0. 05). Theproportion of CT + CC genotype at the 9 loci of Mn-SOD gene in diabetes nephropathy was 40. 00 % ,the proportion of CT + CC genotype at position 5 777 of the Mn-SOD gene was 98. 00 % . The expression level of miR-495-3p in diabetes nephropathy patients with T2DM duration ≥ 10 years was(0. 46 ± 0. 14), which was significantly lower than that in patients with T2DM duration < 10 years(P< 0. 05). The expression level of miR-495-3p in patients with poor prognosis of diabetes nephropathy was (0. 47 ± 0. 14), which was significantly lower than that in patients with good prognosis(P< 0. 05). The proportion of CT + CC at Mn-SOD gene locus 9 genotype in patients with poor prognosis was 73. 33 % , which was significantly higher than that in patients with good prognosis (P <0. 05). There was no significant difference in CT + CC ratio of Mn-SOD gene 5 777 genotype betweenpatients with poor prognosis and those with good prognosis ( P > 0. 05). The area under the ROCcurve for predicting poor prognosis of miR-495-3p and Mn-SOD gene 9 locus genotypes was 0. 784(95 % CI: 0. 705 - 0. 863) and 0. 786 (95 % CI: 0. 700 - 0. 872), respectively ( P < 0. 05).Conclusion The level of miR-495-3p in patients with diabetes nephropathy is reduced, miR-495-3p expression and Mn-SOD genetypes have certain application values in predicting the prognosis ofdiabetes nephropathy.

Key words: miR-495-3p, manganese superoxide dismutase, diabetes nephropathy, prognosis