Abstract: Objective To analyze the diagnostic value of miR-425-5p in endometrial cancer(EC) and the effect of inhibiting miR-425-5p on the proliferation, migration, and apoptosis of ECcells. Methods The expression level of miR-425-5p in serum and tissues of patients with EC andbenign uterine lesions was detected by RT-qPCR, and was compared among patients with differentpathological characteristics of EC. The relationship between miR-425-5p expression in the tissues andserum and the pathological characteristics was analyzed, as well as its diagnostic value for EC. Theexpression level of miR-425-5p in normal endometrial epithelial hEEC cells, endometrial carcinomaIshikawa, HEC-1A, HEC-1B cells was detected by RT-qPCR. Ishikawa cells were divided into 3groups: Con group ( no treatment control), anti-miR-NC group ( transfected with anti-miR-425-5p) and anti-miR-425-5p group (transfected with anti-miR-425-5p). RT-qPCR was used to detect the miR-425-5p expression level. MTT and flow cytometry were used to detect cell proliferation, cell cycle and apoptosis. cell migration was detected by Transwell. The expression levels of CyclinD1,PCNA, Bax, Bcl-2 and MMP9 were detected by Western blotting. Results The expression level ofmiR-425-5p in serum and tissues of the endometrial carcinoma group was higher than that of the benign lesion group (P< 0. 05). There were statistically significant differences when comparing the expression level of miR-425-5p in patients with different lymph node metastasis statuses, FIGO stagesand degrees of differentiation (P< 0. 05). The expression of miR-425-5p in tissues and serum was positively correlated with FIGO stage, lymph node metastasis, and degree of differentiation ( r =0. 793, 0. 689, 0. 665, 0. 694, 0. 652, 0. 672, P < 0. 05). The AUC of miR-425-5p in tissuesand serum for the diagnosis of EC were 0. 782 and 0. 762, respectively. The sensitivities were74. 36 % , 71. 79 % , specificity, and the specificities were 71. 79 % , 70. 51 % , respectively. The expression level of miR-425-5p in endometrial carcinoma Ishikawa, HEC-1A, HEC-1Bcells was increased when compared with that in hEEC cells ( P < 0. 05). The expression level ofmiR-425-5p, cell activity, the proportion of cells in the S phase, the number of migrated cells,and the expression levels of Bcl-2, MMP9, CyclinD1 and PCNA proteins in the anti-miR-425-5pgroup were significantly decreased when compared with those in the anti-miR-NC group or the Congroup, while the proportion of cells in the G0 / G1 phase, the apoptosis rate and the protein expression of Bax were significantly increased (P < 0. 05). Conclusion miR-425-5p is highly expressedin EC tissues and serum, its expression level in EC tissues and serum is related to the patients’FIGO stage, lymph node metastasis and degree of differentiation. It has certain values in the diagnosisof EC. Moreover, inhibition of miR-425-5p can significantly inhibit the proliferation and migration ofEC cells, and induce cell apoptosis.

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