Journal of Medical Molecular Biology ›› 2024, Vol. 21 ›› Issue (1): 17-24.doi: 10.3870/j.issn.1672-8009.2024.01.003
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Abstract: Objective To explore the effect of miR-126-5p on apoptosis and inflammatory response in mouse hippocampal neuron HT22 cells mediated by oxygen-glucose deprivation / reperfusion (OGD / R) by targeting tumor necrosis factor receptor-associated factor 3 (TRAF3). Methods The OGD / R cell model was established in vitro by simulating ischemia / reperfusion (I / R) injury. The targeting relationship between miR-126-5p and TRAF3 and the effect of miR-126-5p on apoptosis and inflammatory response in HT22 cells were analyzed. Results The level of miR-126-5p inthe OGD / R group was down-regulated while the mRNA and expression levels of TRAF3 were upregulated when compared with those in the control group. The cell survival rate and the Bcl-2 expression level in the OGD / R group were decreased while the lactate dehydrogenase ( LDH) release, the apoptosis rate and the protein expression levels of Bax and cleaved caspase-3 in the OGD / Rgroup were increased when compared with those in the control group (all P< 0. 05). The protein expression levels of TRAF3, Bax and cleaved caspase-3, the LDH release, the apoptosis rate were significantly decreased in the OGD / R + miR-mimic group and the OGD + miR-mimic + pcDNA group while the cell survival rate and the protein expression level of Bcl-2 were increased when compared with those in the OGD / R + mimic-NC group. In addition, the above indicators were elevated in the OGD + miR-mimic + pcDNA-TRAF3 group while the cell survival rate was significantly lowered when compared with those in the OGD/R+mimic-NC group (all P<0.05). Conclusion miR-126-5p can inhibit the OGD/R-mediated apoptosis of and inflammatory response in HT22 cells by targeting TRAF3, thus playing a protective role on neuronal cells.
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R741. 02" target="_blank"> R741. 02
R587. 1
ZHAO Li, ZHAO Lei, XIE Ailing, WANG Yamei, WU Yujuan, TANG Shuang. miR-126-5p Inhibits Oxygen-glucose Deprivation / Reperfusion Mediated Apoptosis and Inflammatory Response in HT22 Cells by Targeting TRAF3 #br#[J]. Journal of Medical Molecular Biology, 2024, 21(1): 17-24.
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URL: http://tjqk.magtech.com.cn/yxfzswx/EN/10.3870/j.issn.1672-8009.2024.01.003
http://tjqk.magtech.com.cn/yxfzswx/EN/Y2024/V21/I1/17