Journal of Medical Molecular Biology ›› 2023, Vol. 20 ›› Issue (5): 426-431.doi: 10.3870/j.issn.1672-8009.2023.05.008

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Effect of LncRNA MALAT1 on Lipopolysaccharide-induced Kupffer Cell Proliferation and Inflammatory Factor Expression via Regulation of miR-181d-5p

  

  1. 1Department of Infectious Diseases, Suizhou Central Hospital, Suizhou Hospital Affiliated to Hubei University of Medicine, Suizhou, Hubei, 441300, China  2Department of Gastroenterology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei, 441021, China
  • Online:2023-09-30 Published:2023-11-13

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Abstract: Objective To investigate the effect of LncRNA MALAT1 on lipopolysaccharide (LPS) -induced survival and expression of inflammatory factors in kupffer cells ( KCs) and its possible mechanism. Methods LPS-induced KCs were used to establish a cell injury model and were randomly divided into 8 groups: Con group, LPS group, LPS + si-NC group, LPS + si-MALAT1 group, LPS + miR-NC group, LPS + miR-181d-5p group, LPS + si-MALAT1 + anti-miR-NC group, LPS + si-MALAT1 + anti-miR-181d-5p group. The expression levels of LncRNA MALAT1 and miR-181d-5p were detected by qRT-PCR. Cell viability was detected by CCK-8 method. The levels of IL-6, IL-12 and TNF-α were detected by ELISA. The targeting relationship between LncRNA MALAT1 and miR-181d-5p was detected by dual luciferase gene reporter assay. Results The expression of LncRNA MALAT1 in the LPS group was increased (P< 0. 05), the levels of IL-6, IL12 and TNF-α were increased (P < 0. 05), while the expression of miR-181d-5p was decreased (P< 0. 05), and the cell viability was decreased (P < 0. 05), when compared with those in the Con group. The cell survival rate in the LPS + si-MALAT1 group was increased (P < 0. 05), while the levels of IL-6, IL-12 and TNF-α were decreased (P< 0. 05), when compared with those in the LPS + si-NC group. The LncRNA MALAT1 negatively regulated the expression of miR-181d-5p. The cell survival rate in the LPS + miR-181d-5p group was increased (P < 0. 05), while the levels of IL-6, IL-12 and TNF-α were decreased (P< 0. 05), when compared with those in the LPS + miR-NC group. The survival rate in the LPS + si-MALAT1 + anti-miR-181d-5p group was decreased (P< 0. 05), while the levels of IL-6, IL-12, TNF-α were increased (P< 0. 05), when compared with those in the LPS + si-MALAT1 + anti-miR-NC group. Conclusion Interfering with the expression of LncRNA MALAT1 could promote the survival of LPS-induced KCs and inhibit the expression of inflammatory factors by promoting the expression of miR-181d-5p.

Key words: LncRNA MALAT1, miR-181d-5p, lipopolysaccharide, kupffer cells, inflammatory factors

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