Abstract: Objective To explore the effects of miR-485 on cerebral ischemia-reperfusion (CI/ R) injury and oxygen glucose deprivation / resupply ( OGD/ R) induced apoptosis in BV2 cells. Methods The CI/ R injury rat model was established by thread embolism method, rats were then injected with agomir-485 through caudal vein to observe their brain injury at 24 h time point after reperfusion. miR-485 overexpressed BV2 cell line was constructed, cells were then induced with OGD/ R to observe cells apoptosis and oxidative stress in vitro. Results The results of in vivo experiment showed that the expression level of miR-485 in brain tissues of CI/ R rats was decreased, and agomir-485 intervention could alleviate the neurological damage, reduce the level of Bax / Bcl-2 and the activities of LDH and GSH-px, and increase the activity of SOD in CI/ R rats. The results of in vitro experiment showed that OGD induction could reduce the expression level of miR-485. The overexpression of miR-485 could increase SOD activity, reduce apoptosis rate and the activities of LDH and GSH-px. Conclusion Overexpression of miR-485 can relieve CI/ R injury and reduce the OGD/ R induced apoptosis in BV2 cells, the mechanism may be related to the regulation of oxidative stress.

Key words: cerebral ischemia-reperfusion injury, oxygen glucose deprivation / resupply, miR-485, oxidative stress, apoptosis