Abstract: Objective To investigate the inhibitory effect of recombination plasminogen Kringle 5 (K5) on angiogenesis in mice. Methods Scratch assay and adhesion ability assay were used to determine the effect of recombinant K5 on the migration and adhesion of in vitro cultured mouse endothelial cells. The effect of K5 on angiogenesis was detected by utilizing in vitro angiogenesis system, wound healing and chicken embryo allantoic membrane ( CAM) angiogenesis ability assay. Results K5 (200 nmol / L) markedly inhibited the migration and adhesion of endothelial cells as well as the growth of new blood vessels. K5 also significantly inhibited wound healing in mice at the concentration of 2 to 18 mg / kg. In addition, 6, 12 and 25 mg / L K5 significantly inhibited CAM neovascularization, with the strongest inhibitory effect of K5 at 25 mg / L. Conclusion These results suggest that K5 can significantly inhibit the angiogenesis of vascular endothelial cells, which provides a theoretical basis for clinical treatment of angiogenesis

Key words: human plasminogen Kringle 5, endothelial cells, angiogenesis