Effect of miR-214-5p on DNA Damage and Chemotherapy Sensitivity in Neuroblastoma Cells by Regulation of FANCA

doi:10.3870/j.issn.1672-8009.2025.06.004

Journal of Medical Molecular Biology ›› 2025, Vol. 22 ›› Issue (6): 557-562.doi: 10.3870/j.issn.1672-8009.2025.06.004

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Effect of miR-214-5p on DNA Damage and Chemotherapy Sensitivity in Neuroblastoma Cells by Regulation of FANCA

ZHAO Conghui¹, LIU Bing², LIU Jiajia³, CUI Ling⁴, LIU Xiaoxia⁵, LEI Jianhua²

¹Department of Rehabilitation Medicine,²Department of Neurology,³the Fifth Department of Neurosurgery,⁴the First Department of Radiotherapy,⁵the Second Department of Immunology,Handan Central Hospital,Handan,Hebei,056000,China

Received:2025-04-24 Online:2025-11-30 Published:2025-12-25
Contact: LIU Bing(E-mail:13784221106@163.com)
Supported by:
Medical Science Research Project of Hebei Province(No.20241660)

Abstract

Abstract: Objective To explore the effect and mechanism of microRNA-214-5p(miR-214-5p)on DNA damage and chemotherapy sensitivity in neuroblastoma(NB). Methods The relationship between Fanconi anemia complementary group A (FACNA) protein and clinicopathological characteristics of NB was analyzed by TARGET database.NB cell lines with low expression of miR-214-5p and overexpression/low expression of FANCA were constructed,and their effects on the proliferation of NB in vivo and in vitro were detected by cell counting kit-8(CCK-8)and transplanted tumor model.The targeted relationship between miR-214-5p and FANCA was verified. Results FANCA was screened out as the intersection gene correlated with NB in GEO and TARGET databases,and its expression level was related to cells proliferation pathways,and the prognosis and clinicopathological characteristics of NB.Compared with those in the empty vector(Vector)group,the cells viability,and the volume and weight of transplanted tumors were increased in the FANCA group(P<0.05).Compared with those in the shNC group,the cells viability was decreased in the shFANCA1/2 groups(P<0.05).Compared with those in the control group,the phosphorylated histone γ-H2AX was increased after treated with 1 mmol/L H₂O₂(P<0.05),but γ-H2AX was decreased after FANCA overexpression(P<0.05).With the increase of doxorubicin concentration,cells viability was decreased,but it was higher in the FANCA group than in the Vector group(P<0.05).There was a targeted relationship between miR-214-5p and FANCA. Conclusion miR-214-5p can inhibit the proliferation of NB cells by reducing FANCA expression level,and regulate the DNA damage and chemotherapy sensitivity in cells.

Key words: neuroblastoma, microRNA-214-5p, fanconi anemia complementary group A protein, DNA damage, chemotherapy sensitivity

CLC Number:

R739.4

ZHAO Conghui, LIU Bing, LIU Jiajia, CUI Ling, LIU Xiaoxia, LEI Jianhua. Effect of miR-214-5p on DNA Damage and Chemotherapy Sensitivity in Neuroblastoma Cells by Regulation of FANCA[J]. Journal of Medical Molecular Biology, 2025, 22(6): 557-562.

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Effect of miR-214-5p on DNA Damage and Chemotherapy Sensitivity in Neuroblastoma Cells by Regulation of FANCA

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