Abstract: Objective To explore the effect and mechanism of stearyl coenzyme-A desaturation enzyme 1 (SCD1) on IL-1β induced pancreatic β cell damage. Methods SCD1 gene was inserted into the pEGFP-C1 vector to construct the pEGFP-SCD1 overexpression plasmid. The pancreatic β cell lines INS-1 and βTC-6 were transferred with the pEGFP-SCD1 plasmid and were treated with IL-1β. Western blotting, Tunel staining, and GSIS / KSIS method were used to explore the protective effect of SCD1 on pancreatic β cells. Results IL-1β treatment reduced the expression level of SCD1 in pancreatic β cells. Overexpression of SCD1 reversed the IL-1β induced reduction of insulin synthesis and secretion and the apoptosis in pancreatic β cells. Conclusion SCD1 can protect the pancreatic β cells from cell damage caused by IL-1β.

Key words: SCD1, IL-1β, pancreatic β cells