Effects of Bushen Huatan Decoction on the β2AR/cAMP-PKA-CREB Signaling Pathway in a Rat Model of Postmenopausal Osteoporosis

doi:10.3870/j.issn.1672-0741.25.07.012

Abstract

Abstract: Objective To investigate the effects of Bushen Huatan decoction(BSHT) on the β₂ adrenergic receptor(β₂AR)and the cyclic AMP(cAMP)-protein kinase A(PKA)-cAMP response element binding protein(CREB)signaling pathway in ovariectomized rats. Methods A postmenopausal osteoporosis rat model was established by ovariectomy.30 rats were randomly divided into 3 groups:A sham operation (Sham)group,a model (OVX)group,and a (BSHT)group.5 weeks after the operation,the rats received different treatments(4 mL of normal saline for the Sham and OVX groups,and BSHT for the BSHT group).Micro-computed tomography(micro CT)was used to assess the microstructure of the femur.The mRNA and protein expression levels of β₂AR,PKA,and CREB in the femur were detected by RT-PCR and Western blotting,respectively.The distribution of the proteins in femur tissue was determined by immunohistochemistry(IHC). Results Compared with the Sham group,the OVX group showed significantly decreased bone mineral density(BMD),bone volume fraction(BV/TV),trabecular thickness(Tb.Th),and trabecular number(Tb.N),as well as increased trabecular separation(Tb.Sp).The mRNA and protein levels of PKA and CREB were also decreased,while the mRNA and protein levels of β₂AR were significantly increased(all P<0.05)in the OVX group.Compared with the OVX group,the BSHT group exhibited significantly increased BMD,BV/TV,Tb.Th,Tb.N,and expression levels of PKA and CREB,along with significantly decreased expression levels of β₂AR(all P<0.05).IHC results showed that β₂AR,PKA,and CREB were positively expressed in the cytoplasm of osteoblasts surrounding the trabeculae and in bone marrow stromal cells. Conclusion BSHT may prevent and treat osteoporosis in ovariectomized rats by regulating the expression of β₂AR and the cAMP-PKA-CREB signaling pathway.

Key words: Bushen Huatan decoction, β₂AR, cAMP-PKA-CREB signaling pathway, postmenopausal osteoporosis

CLC Number:

R285.5

Zhang Lin, Chen Zhihao, Yang Shuoshuo et al. Effects of Bushen Huatan Decoction on the β₂AR/cAMP-PKA-CREB Signaling Pathway in a Rat Model of Postmenopausal Osteoporosis[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(2): 220-225.

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Effects of Bushen Huatan Decoction on the β₂AR/cAMP-PKA-CREB Signaling Pathway in a Rat Model of Postmenopausal Osteoporosis

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