Effect of Panx1 Regulating ATP Release on Apoptosis of Cisplatin-resistant SKOV3/DDP Cells in Human Ovarian Cancer

doi:10.3870/j.issn.1672-0741.25.07.034

Abstract

Abstract: Objective To investigate the expression of Panx1 in cisplatin-resistant SKOV3/DDP strains of human ovarian cancer and its mechanism in drug resistance. Methods Western blotting was used to detect the expression of Panx1 in SKOV3 and SKOV3/DDP.Flow cytometry was used to detect the apoptosis of SKOV3/DDP cells,and the expression of Panx1 in SKOV3/DDP cells and its effect on apoptosis were analyzed.SKOV3/DDP overexpression plasmid was constructed,and SKOV3/DDP cells were transfected successfully.Apoptosis was detected by flow cytometry.ATP content in cell supernatant was detected by ELISA to analyze the role and mechanism of Panx1 mediating SKOV3/DDP resistance. Results The expression of Panx1 protein in SKOV3/DDP was significantly lower than that in SKOV3 group(0.17±0.05 vs.0.35±0.03,P<0.01).The cis-diamine dichloroplatinum(DDP)could significantly inhibit SKOV3 cell proliferation in a time-and dose-dependent manner.Subsequent experiments were conducted at 36 h and 32 μmol/L.The apoptosis rate of SKOV3 and SKOV3/DDP groups was significantly increased after DDP treatment(33.96%±0.03% vs.5.01%±0.00%,P<0.01;14.66%±0.02% vs.4.65%±0.01%,P<0.01).The apoptosis rate of SKOV3/DDP overexpression plasmid group was significantly higher than that of untreated group(15.69% vs.4.10%,P<0.01).ATP content in extracellular fluid of SKOV3/DDP overexpression plasmid group was significantly higher than that of control group(33.57±6.00 vs.10.65±0.76 ng/mL,P<0.01). Conclusion Up-regulation of Panx1 can increase apoptosis of SKOV3/DDP cells by increasing ATP release.Cisplatin resistance of ovarian cancer may be related to the decrease of ATP release mediated by low expression of Panx1.

Key words: ovarian cancer, Panx1, cisplatin resistance, ATP, apoptosis

CLC Number:

R737.31

Long Shenggen, Shu Kuanyong, Zhu Qizhou et al. Effect of Panx1 Regulating ATP Release on Apoptosis of Cisplatin-resistant SKOV3/DDP Cells in Human Ovarian Cancer[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(3): 327-332.

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