The Role of LHPP in Breast Cancer Cell Function and Immune Regulation

doi:10.3870/j.issn.1672-0741.25.09.045

Abstract

Abstract: Objective To investigate the expression level of LHPP in human breast cancer and its impact on proliferation and migration and to conduct a preliminary exploration of its immunoregulatory function. Methods The expression level of the LHPP protein was examined in breast cancer patients.To manipulate LHPP expression,breast cancer cells were transfected with overexpression plasmids (T47D) or specific shRNAs(MCF7).LHPP protein levels,along with cell proliferation,migration capacity,and Akt pathway activity,were quantified via Western blotting,CCK-8,Transwell,and related assays,respectively.A preliminary investigation was conducted to examine the role of LHPP in immune regulation. Results The expression was significantly lower in cancer tissues than in adjacent non-tumor tissues from breast cancer patients(P<0.05).LHPP expression varied across cancer cell lines,with T47D showing lower levels and MCF7 exhibiting relatively higher levels(all P<0.05).In T47D cells,LHPP overexpression successfully led to increased levels of LHPP.As a result,cell proliferation,migration,and the p-Akt/Akt ratio were significantly decreased(all P<0.05).In MCF7 cells,shLHPP transfection knocked down LHPP expression,leading to significant changes in the above-mentioned phenotypic and pathway markers(all P<0.05). Conclusion LHPP,which is expressed at low levels in breast cancer,functions as a tumor suppressor by inhibiting cancer cell proliferation and migration.This effect may be mediated through the Akt signaling pathway.

Key words: LHPP, breast cancer, cell proliferation, cell migration, Akt, microenvironment

CLC Number:

R737.9

Xiang Min, Ge Yanfeng, Jiang Yifan et al. The Role of LHPP in Breast Cancer Cell Function and Immune Regulation[J]. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong, 2026, 55(1): 97-103.

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